ABSTRACT
Se presentó a la Cátedra de Endodoncia de la Facultad de Odontología de la Universidad de Buenos Aires un paciente masculino de 62 años de edad que al examen clínico presentaba una fístula vestibular en la zona de la pieza 1.2 y dolor a la percusión. Al examen radiográ-fico se identificó una lesión apical extensa abarcando las piezas dentarias 1.2 y 1.1 endodónticamente trata-das con alteración severa de la anatomía del espacio endodóntico, así como la presencia de postes metáli-cos que no respetaban el eje del canal radicular. Ante el análisis tomográfico se observó una perforación de la pieza 1.2 y una lesión periapical extensa afectando ambas corticales (vestibular y palatina). Se decidió un abordaje microquirúrgico con técnicas de regenera-ción ósea guiada (ROG) y se realizaron los controles clínico-tomográficos a los 6, 12 y 24 meses. Por otro lado, se evaluó con micromografía de rayos X la ana-tomía de los ápices radiculares resecados. La lesión extirpada fue analizada histológicamente (AU)
A 62-year-old male patient attended the Endodontics department of the Buenos Aires University. He was examined clinically and a vestibular fistula in 1.2 area and pain under percussion were found. Radiographic examination identified an extended periapical lesion compromising teeth 1.2 and 1.1 with endodontic treatment severely altering the root canal anatomy, as well as metallic cast posts that did not preserve root canal axis. Regarding the tomographic analysis, a vestibular root perforation was observed (1.2), and both, vestibular and palatal corticals, were affected. We decided to perform a surgical approach with guided bone regeneration techniques (GBR). Clinical-CBCT controls were done at 6, 12 and 24 months. Furthermore, the anatomy of the resected root apex-es was evaluated with X ray microtomography. The removed lesion was histologically analyzed (AU)
Subject(s)
Humans , Male , Middle Aged , Periapical Periodontitis/surgery , Argentina , Schools, Dental , Cone-Beam Computed Tomography/methods , Membranes, ArtificialABSTRACT
Objectives: This study aimed to evaluate and compare the mechanical properties of platelet?rich fibrin (PRF) membrane with that of commercially available collagen membranes and chorionic membranes. Materials and Methods: The modulus of elasticity and hardness of PRF membrane, bovine collagen membrane, fish collagen membrane, and chorionic membrane were assessed using a universal testing machine. The in vitro degradation rate was assessed by placing these membranes on a temperature?controlled shaker set for one week. The degradation profiles were expressed as the accumulated weight loss of the membrane. A scanning electron microscope (SEM) evaluation of these membranes was done under both low and high magnification. One?way analysis of variance (ANOVA) and Tukey’s post hoc tests were performed for statistical analysis. Results: A statistically significant difference in the tensile strength and hardness of membranes was observed. Bovine collagen membrane had the highest strength (84.11 MPa and 16.46 MPa) followed by fish collagen membrane, chorionic membrane, and least for PRF membranes observed. The degradation rate at one week was highest for the PRF membrane (55.6%), followed by the fish collagen membrane (32.5%). SEM evaluation showed that the bovine collagen membrane had significantly higher numbers of collagen fibres compared to the fish collagen membrane and chorionic membrane. Conclusion: Bovine collagen membrane had the highest mechanical properties with the maximum amount of collagen fibre meshwork. Only the PRF membrane had cellular distribution in its composition, while the commercially available membrane had significantly higher numbers of collagen fibres with the total absence of cellular components
ABSTRACT
@#Tooth loss is accompanied by alveolar bone absorption or defect, resulting in insufficient bone and soft tissue. In addition to restoring the masticatory function of missing teeth, implant treatment should also needs to restore the contour and shape of the dental arch. Guided bone regeneration is a common means of bone increase. Xenogeneic granular bone substitute materials are widely used in the field of clinical bone augmentation due to their advantages of long degradation time and low immunogenicity, but other problems, such as inconvenient operation and low osteogenic activity, remain. Plasmatrix can effectively improve the effect of oral tissue regeneration and reduce the occurrence of postoperative complications, and its application in oral tissue regeneration is gradually increasing. This article first introduces the main application forms of plasmatrix in horizontal bone augmentation (mainly solid plasmatrix membrane and plasmatrix bone block), and reclassifies horizontal bone defects according to commonly used decision-making schemes in clinical bone augmentation, in other words, whether the implant can be placed in the ideal position and whether there is bone dehiscence after implantation. Type Ⅰ defects refers to the situation where the bone at the implant site can allow the insertion of an implant with ideal size, and there is no bone dehiscence around the implant, but the alveolar bone contour is not ideal; type Ⅱ defects refers to the situation that when an ideal size implant is placed at the implant site determined by the future prosthesis position, there will be bones on three sides of the implant, but there is bone dehiscence in the buccal bone wall (the length of bone dehiscence is less than 50% of the implant length); type Ⅲ defects refers to the situation where the bone volume at the implant site is not enough to for the placement of the ideal size implant at the ideal position, and bone grafting is required to restore the bone volume before the implant placement. The application of plasmatrix in different types of bone defects is then described. In type Ⅰ bone defects, the solid plasmatrix membrane is used instead of the collagen membrane; in type Ⅱ bone defects, the bone defect around the implant is filled by plasmatrix bone block and then covered with collagen membrane and solid plasmatrix membrane; and in type Ⅲ bone defects, plasmatrix bone block is used to replace autogenous bone block to fill the defect area, and titanium screws are used for fixation. The defect is then covered with a collagen membrane and a solid plasmatrix membrane. This article aims to provide oral clinicians with a comprehensive understanding of plasmatrix and simplify the guidelines for bone regeneration operations.
ABSTRACT
@#Vertical bone augmentation surgery still faces considerable challenges in clinical practice due to various problems, such as difficulty in restoring the ideal alveolar bone height and biological complications, and because it is highly technically sensitive. Plasmatrix is derived from patients’ own blood, and it can effectively promote the vascularization of the regenerated area, recruit stem cells, and reduce inflammation when used in vertical bone augmentation. Based on studies published worldwide, this article first divides vertical bone augmentation into 3 categories according to the height of the expected alveolar ridge, namely, type Ⅰ, the required vertical bone gain is less than 4 mm; type Ⅱ, the required vertical bone gain is between 4-8 mm; and type Ⅲ, the required vertical bone gain is greater than 8 mm. In the type Ⅰ vertical bone augmentation, the plasmatrix bone block is directly placed in the defect area and covered with the plasmatrix membrane before tension-free suturing; in the type Ⅱ vertical bone augmentation, the plasmatrix bone block should be placed in the defect area and fixed with titanium nails and then covered with an absorbable collagen membrane and plasmatrix membrane with a tension-free suture; in the type Ⅲ vertical bone augmentation, additional active ingredients (such as bone morphogenetic protein, autologous bone, etc.) should be added to the plasmatrix bone block and strong fixation (such as titanium nails) should be used. Absorbable collagen and plasmatrix membranes should be used to cover the surface of the bone block, and the flap should be sutured. According to different types of vertical bone augmentation categories, the above methods optimize the vertical bone augmentation effect. This article aims to provide a reference and guidelines for oral clinicians to fully understand plasmatrix and simplify the classification and operation of vertical bone augmentation.
ABSTRACT
Objective@#To evaluate the osteogenic effect of concentrated growth factor (CGF) combined with deproteinated bovine bone mineral (DBBM) in site preservation using clinical and histomorphometric observations.@*Methods @#A total of 26 patients who needed extraction of affected teeth and received staged implantation after site preservation were selected. The patients were randomly divided into the DBBM group (Bio-Oss was implanted simultaneously after extraction) and CGF+DBBM group (CGF+Bio-Oss was implanted simultaneously after extraction), with 13 patients in each group, and both groups were covered with Bio-Gide collagen membrane. Cone beam computed tomography (CBCT) was performed preoperatively and 6 months later to measure the changes in alveolar bone height and width, and the bone specimens were drilled 6 months after site preservation during implant surgery for histological analyses.@*Results@# CBCT showed that the height and width of alveolar bone were absorbed 6 months after site preservation in the CGF+DBBM and DBBM groups, and the reduction in alveolar ridge width in the CGF+DBBM group was statistically less than the DBBM group (P <0.05). The histomorphometry results showed that the percentage of new bone in the CGF+DBBM and DBBM groups were 35.30% ± 3.56% and 26.38% ± 5.04%, respectively, and the amount of new bone in the CGF+DBBM group was statistically higher than the DBBM group (P<0.05). @*Conclusion @#CGF combined with DBBM is superior to DBBM in maintaining the alveolar bone volume and shape in site preservation, which creates favorable conditions for implant restoration.
ABSTRACT
BACKGROUND: Guided bone regeneration technology, as a most widely used method for repairing bone defects, has been extensively used in the field of stomatology. However, there are few reports on the guided bone regeneration technology in long bone defects. OBJECTIVE: To explore the effects of guided bone regeneration combined with kidney-tonifying therapy on the repair of femoral bone defects in rats, and investigate its osteogenic efficacy and underlying mechanism. METHODS: Thirty-six Sprague-Dawley rats were randomly divided into six groups: blank group, guided bone regeneration group, high-, moderate-, and low-dose kidney-tonifying groups, and ossotide tablets group. The femur bone defect model of rats was established, and was treated by guided bone regeneration except for blank group. Bio-Gide collagen membrane combined with autologous bone was implanted by guided bone regeneration. The kidney-tonifying groups were given 0.216, 0.108 and 0.054 g/(kg•d) Qianggu capsule via gavage for 8 weeks. The ossotide tablets group was given 0.58 mg/(kg•d) ossotide tablets via gavage for 8 weeks. At 12 weeks after surgery, the osteogenesis was evaluated by X-ray examination, hematoxylin-eosin staining and Masson staining of bone tissue. The mRNA expression levels of alkaline phosphatase, Runx-2, vascular endothelial growth factor and bone morphogenetic protein-2 in bone tissues were detected by quantitative real-time RT-PCR. RESULTS AND CONCLUSION: Results of X-ray examination and hematoxylin-eosin staining and Masson staining of bone tissue showed that the scores of Lane Sandhu and Huddleston in each group were significantly higher than those in the blank group (P < 0.001). The scores in the high-and moderate-dose kidney-tonifying groups were significantly higher than those in the guided bone regeneration group (P < 0.01). RT-PCR results showed that the mRNA expression levels of alkaline phosphatase, Runx-2, vascular endothelial growth factor and bone morphogenetic protein-2 in bone tissue in the high-and moderate-dose kidney-tonifying groups were significantly higher than those in the blank group (P < 0.01), and were superior to the guided bone regeneration group (P < 0.05). In summary, guided bone regeneration combined with kidney-tonifying can significantly promote the repair of femoral bone defects, reduce bone absorption and improve osteogenic efficacy in rats. The mechanism of promoting bone regeneration and angiogenesis may be by up-regulating the expression of related osteogenic factors and angiogenic factors in the environment where the membrane barrier creates a dominant growth of bone tissue.
ABSTRACT
Guided bone regeneration (GBR) is an important technique to solve bone defect problems. In this technique, GBR barrier membranes play an irreplaceable role. GBR membranes can act as a barrier protecting fibroblasts from bone defects and promote osteoblast adhesion and proliferation, leading to bone regeneration. GBR barrier membranes should be enhanced because of the disadvantages of collagen membranes, which are extensively applied to the field of GBR. Therefore, various efforts have been devoted to modifying the antibacterial and osteogenic properties of GBR barrier membranes and developing novel materials. This article reviews the research advancements on the modification of GBR barrier membranes and discover future directions for the development of GBR barrier membranes to provide a reference for bone tissue engi-neering and repair.
Subject(s)
Bone Regeneration , Collagen , Membranes, Artificial , Osteoblasts , OsteogenesisABSTRACT
Objective@#To study the vascularization of the collagen-binding basic fibroblast growth factor (bFGF)-loaded collagen membranes in soft tissue repair in the hard palates of rats. @*Methods@#Ninety-six male 6-week-old Wistar rats were randomly divided into 4 groups, and 3-mm-diameter circular soft tissue defects were produced from the distal surface of the third molar to the mesial surface of the first molar in their hard palates. The defects were covered with a collagen-targeting bFGF/collagen membrane, a free bFGF/collagen membrane, a collagen membrane or no membrane (control group). Every 6th rat was randomly sacrificed at 1, 2, 4 and 8 weeks in every group after surgery. Wound healing and the number of new blood vessels were measured by hematoxylin-eosin (HE) staining. @*Results @#The numbers of new blood vessels in the collagen-targeting bFGF/collagen membrane group were 8.94 ± 0.61, 17.39 ± 2.08 and 11.22 ± 1.66 at 1, 2 and 4 weeks after surgery, respectively, which were significantly greater than the values in the other groups (P<0.05). At 8 weeks, the number of new blood vessels in the collagen-targeting bFGF/collagen membrane group was 4.17 ± 1.28, and there was no significant difference in the numbers of new blood vessels between any of the groups (P > 0.05).@*Conclusion@#Collagen-targeting bFGF/collagen membranes had a favorable effect on promoting angiogenesis during wound healing, and promoted wound healing.
ABSTRACT
Methods: Twenty Sprague Dawley rats were randomly divided into 2 groups ( n=10). DermalGen were implanted subcutaneously into the dorsum of rats in experimental group, and the rats in control group were treated with sham-operation. At 3, 7, and 15 days and 1, 3, 6, and 12 months after operation, the samples of experimental group were harvested and gross observation, histological observation, CD31 immunohistochemical staining, and transmission electron microscope observation were taken to observe the inflammatory reaction, angiogenesis, and collagen arrangement. The skin tissues of the control group at 12 months were observed and compared.
ABSTRACT
Objective Studies have shown that low concentrations of nicotine can promote neovascularization and promote wound healing.This article aimed to investigate the influence of low concentration collagen membrane slow-release system on the hard palate trauma of rats.Methods Using poly(lactic acid-co-glycolic acid) (PLGA) copolymer as carrier materials, low concentration nicotine sustained-release particles were prepared by emulsion evaporation method (w/o/w), using collagen membrane as the brace and establish a low concentration collagen membrane system.48 Wistar rats were divided into experimental group and blank group, 3 mm diameter circular wound was made in the forepart palate.Low concentration of nicotine collagen membrane sustained-release particle system and blank collagen membrane (control) were sutured on the wound with 6-0 absorbable thread.Then, observed the wound healing of 0, 3, 7, 10 days and compared the healing differences between each groups.Results Under the electron microscope, the nicotine sustained-release particles were circular, similar size with rough surface, the average diameter were 3.0±0.2μm, the encapsulation efficiency and drug loading rate was 50.2% and 4.12% respectively.In vitro, nicotine sustained-release particles released much more nicotine on the first day, less on the second day, tends to stable and fluctuate within a certain range from the third day on, and declined sharply after about 10 days, nicotine concentration from 3rd to 10th day was fluctuate within 10-5-10-4mol/L.Postoperative wound healing, no significant difference in 3 days(P>0.05), after 7 days, the wound healing of experimental group significantly greater compared with the control (P=0.015).The wound was healed in 10 days after operative, there was no significant difference between two groups(P>0.05).The epithelial proliferation in the experimental group was significantly greater than that in the blank group, there were many fibroblasts, inflammatory cells and new capillaries, the epithelial process is short, the submucosa is loose, and a large number of collagen fibers are produced.The lamina propria is closely connected with the periosteum, and the wound is healed Conclusion Low concentration of nicotine sustained-release particles collagen membrane system may promote wound healing in the hard palate mucosa of rats.
ABSTRACT
Objective:To investigate the feasibility of construction of tissue engineered cartilage by co-culture of bone marrow mesenchymal stem cells (BMSCs) and costal chondrocytes (CCs),and to provide theoretical basis and experimental basis for clinical repair of articular cartilage defects by Wuzhishan miniature pig knee cartilage defects with co-cultured cells.Methods:Density gradient centrifugation method was used to isolate BMSCs from Wuzhishan miniature pig.The double enzyme digestion method was used to isolate CCs.The passage 3 generation of BMSCs and passage 2 generation of CCs were randomly divided into 3 groups:a co-culture group of BMSCs∶CCs for 1∶2 (Group A),a simple CCs (Group B),and a simple BMSCs (Group C).The cell growth curve was drawn,and the content of glycosaminoglycan (GAG) of external separation in chondrocytes was determined.The 12 Wuzhishan miniature pigs were randomly divided into a co-culture cells/collagen membrane experimental group,a collagen membrane control group and the blank group.In the co-culture cells/collagen membrane experimental group,the co-cultured cells/collagen membrane were implanted into the cartilage defects of the mandibular condyle;in the collagen membrane control group,only collagen membrane was implanted;while in the blank group,nothing was implanted.Six animals were sacrificed at 8 and 16 weeks after surgery respectively (2 animals in each group).General observation,cartilage histological score and histopathological examination were carried out.Results:The BMSCs and co-culture cells grew well.The biological activity of CCs was good.After 16 weeks of operation,the repair tissues in the co-cultured cells/collagen membrane experimental group showed hyaline cartilage features:smooth,flat,and integrated well with the surrounding cartilage and subchondral bone.The collagen membrane in the collagen membrane control group was fibrously repaired.Repair tissue gross score in the co-culture cells/collagen membrane experimental group was significantly better than that in the collagen membrane control group and the blank group (both P0.05).Conclusion:BMSCs,CCs and co-cultured cells can function as the seed cells for cartilage tissue engineering,and the co-culture cells (BMSCs∶CCs=1∶2) possess more advantages;the short-term effect of co-culture cells with collagen membrane on repairing cartilage defects is satisfied.
ABSTRACT
Objetivo: determinar la actividad de un injerto basado en el cocultivo de fibroblastos gingivales y queratinocitos en membrana de colágeno comercial Mem-Lok(R) (BioHorizons), Alabama, Estados Unidos) en el tratamiento de recesiones gingivales. Materiales y métodos. Esta investigación fue descriptiva y de diseño experimental. La muestra se conformó de 10 ratas Sprague Dawley a las que se indujeron recesiones gingivales. A 8 de ellas se les aplicó el injerto y las 2 restantes no recibieron tratamiento. Resultados: el análisis descriptivo de los resultados determinó la posibilidad de obtener un cocultivo celular. Luego de la aplicación del injerto, las características clínicas periodontales indicaron salud, consistencia firme, textura a manera de puntillado, contorno festoneado, biotipo grueso, sondaje periodontal de 1 mm y posición de la encía a nivel del límite amelocementario. Conclusiones: el injerto aplicado logró una cobertura radicular del 100 por ciento en todos los casos. No se observó sangrado ni contracción cicatrizal.
Aim: to determine the effectiveness of a graft based onco-cultivation of gingival fibroblast and keratinocytes in commercial collagen membrane Mem-Lok® (BioHorizons, Alabama,USA) in the treatment of gingival recessions. Materials and methods: This research was descriptiveand experimental in design. The sample was composed of 10 Sprague Dawley rats which were induced gingival recession; two of them were not treated and the graft was applied in eightof them. Results: A descriptive analysis of the results was performed, which showed that it was possible to obtain a cellco-culture. After the application of the graft, clinical periodontal characteristics were observed that indicated health: the consistency was firm, the texture resembled dots, scallopedand knife edge margin, a thick biotype and the depth ofgingival sulcus was 1 mm. Conclusions: The applied graft achieved a 100% radicularcoverage in all cases and no bleeding or scar contractionwas observed.
Subject(s)
Animals , Animals , Rats , Collagen/physiology , Fibroblasts/physiology , Gingival Recession/surgery , Cell Culture Techniques/methods , Epidemiology, Descriptive , Membranes, Artificial , Keratinocytes/physiologyABSTRACT
Aims and objectives: To evaluate and compare the effectiveness of the buccal pad of Fat (BPF) and collagen membrane for the reconstruction of the defects secondary to the resection of the Fibrotic bands in oral submucous fibrosis (OSMF). Materials and methods: 20 patients of OSMF diagnosed clinically with mouth opening less than 25 mm were selected. Patients were divided into group I (BPF) and II (collagen membrane) of 10 patients each. After excision of the fibrotic bands, mouth opening was checked and if it was found to be < 35 mm, then bilateral coronoidectomy was carried out along with extraction of third molars. Results were compared within the parameters of maximal mouth opening (MMO), postoperative pain and duration taken for epithelialization. Result: Study showed statistically insignificant difference in the postoperative mouth opening and pain, significant difference in time taken for epithelisation. Conclusion: Present study indicates both BPF and Collagen membrane are versatile materials for the treatment of OSMF. Collagen membrane is superior to BPF in terms of time taken for epithelisation.
ABSTRACT
BACKGROUND: The aim of this study was to evaluate the clinical outcomes of implants that were placed within the maxillary sinus that has a perforated sinus membrane by the lateral window approach. METHODS: We examined the medical records of the patients who had implants placed within the maxillary sinus that has a perforated sinus membrane by the lateral approach at the Department of Oral and Maxillofacial Surgery of Chonnam National University Dental Hospital from January 2009 to December 2015. There were 41 patients (male:female = 28:13). The mean age of patients was 57.2 ± 7.2 years at the time of operation (range, 20–76 years). The mean follow-up duration was 2.1 years (range, 0.5–5 years) after implant placement. Regarding the method of sinus elevation, only the lateral approach was included in this study. RESULTS: Ninety-nine implants were placed in 41 patients whose sinus membranes were perforated during lateral approach. The perforated sinus membranes were repaired with a resorbable collagen membrane. Simultaneous implant placements with sinus bone grafting were performed in 37 patients, whereas delayed placements were done in four patients. The average residual bone height was 3.4 ± 2.0 mm in cases of simultaneous implant placement and 0.6 ± 0.9 mm in cases of delayed placement. Maxillary bone graft with implant placement, performed on the patients with a perforated maxillary sinus membrane did not fail, and the cumulative implant survival rate was 100%. CONCLUSIONS: In patients with perforations of the sinus mucosa, sinus elevation and implant placement are possible regardless of the location and size of membrane perforation. Repair using resorbable collagen membrane is a predictable and reliable technique.
Subject(s)
Humans , Bone Transplantation , Collagen , Follow-Up Studies , Maxilla , Maxillary Sinus , Medical Records , Membranes , Methods , Mucous Membrane , Retrospective Studies , Surgery, Oral , Survival Rate , TransplantsABSTRACT
The aim of the present research was to compare bone neoformation in bone defects treated with platelet-rich fibrin (PRF) and collagen membrane (CM) at 3 and 5 weeks. For this purpose, two bone defects with a width of 4 mm and depth of 6 mm were created in the left distal femur diaphysis of New Zealand rabbits (n=12). The subjects were randomly allocated into two groups. One of the defects was covered with a platelet-rich fibrin membrane (Centrifuged resorbable autologous blood biopolymer without biochemical modification) or a collagen membrane (gold standard - Neo Mem). The second defect was left uncovered (NC). The rabbits were sacrificed after 3 and 5 weeks (3 rabbits per period). The femur was completely removed and processed histomophometrically. The bone neformation analysis was performed using a differential point-counting method. Data was statistically analyzed (ANOVA, Tukey). The histomorphometric results showed that bone neformation in the defects treated with PRF at 3 weeks was equivalent to the CM (p<0.05). After 5 weeks, bone neformation obtained with PRF was higher than the control group and lower compared with the CM (p<0.05). The conclusion of the present study is that bone neformation in defects treated with PRF showed lower histomorphometric results compared with the one obtained with the collagen membrane and higher when compared with the control defects...
El objetivo de la investigación fue comparar la neoformación ósea de defectos óseos tratados con membrana de fibrina rica en plaquetas (PRF) y membrana de colágeno (CM), a las 3 y 5 semanas para lo cual se crearon dos defectos óseos de 4 mm de diámetro y 6 mm de profundidad en la diáfisis femoral distal izquierda de conejos Nueva Zelanda (n=12). Los animales fueron divididos aleatoriamente en 2 grupos. Uno de los defectos fue cubierto con membrana de fibrina rica en plaquetas (Biopolímero Reabsorbible Autólogo de sangre Centrifugada sin modificación bioquímica) o membrana de colágeno (Gold estándar-Neo Mem,) mientras que el segundo defecto se dejó sin cubrir (NC). Los conejos fueron sacrificados después de 3 y 5 semanas (3 conejos/periodo), se les extrae el fémur por desarticulación, se procesa y evalúa histomorfométricamente. El análisis de la neoformación ósea histológica fue realizado mediante el conteo diferencial de puntos. Los datos fueron analizados estadísticamente (ANOVA, Tukey). Los resultados histomorfométricos mostraron que la neoformación ósea de los defectos tratados con PRF a las 3 semanas fue equivalente a la neoformación obtenida en el grupo control y menor comparado con la neoformación del CM (p<0,05). A las 5 semanas la neoformación ósea obtenida con PRF fue mayor a la neoformación del grupo control y menor a la CM (p<0,05). Concluyendo que la neoformación ósea obtenida en los defectos tratados con PRF mostraron resultados histomorfométricos menores a la neoformación obtenida con colágeno y mayores en comparación a la neoformación de los defectos controles...
Subject(s)
Animals , Rabbits , Collagen/therapeutic use , Fibrin/therapeutic use , Membranes, Artificial , Blood Platelets/physiology , Bone Regeneration/physiology , Analysis of Variance , Femur , Time FactorsABSTRACT
A exigência estética nos dias de hoje está junto, senão à frente, dos processos funcionais em reabilitação oral. A perda dentária precoce de dentes anteriores, quando relacionada a casos de severas perdas ósseas, estão entre os casos mais complexos para se atingir resultados positivos na Implantodontia, seja ela estética ou funcional. No presente caso, o paciente apresentou-se com fratura do elemento incisivo central superior esquerdo, com perda óssea considerável na face vestibular e necessitando de reabilitação com implantes osseointegrados e regeneração óssea guiada (ROG), acompanhado de membrana de colágeno reabsorvível. Após o período de osseointegração, foram realizados procedimentos de moldagem e condicionamento dos tecidos ao redor, através de instalação de provisório e posterior confecção de restauração final metalocerâmica. Após acompanhamento tomográfico do caso durante cinco anos, fica evidente a manutenção tecidual e óssea da região citada acima.
Nowadays, the esthetic demands are well-beyond the functional processes for oral rehabilitation. Premature tooth loss in the anterior zone, when related to severe bone loss, are the most complex cases to generate positive results on implant dentistry. In this case, the patient presented with fracture of the maxillary left central incisor, a considerable buccal bone loss, and in need of a dental implant and guided bone regeneration (GBR) with a resorbable collagen membrane. After the osseointegration period, impression procedures and peri-implant soft tissue conditioning were performed by using a provisional restoration before the defi nitive metalloceramic restoration. The 5-year CBCT follow-up revealed soft and hard tissue stable contours in the aforementioned region.
Subject(s)
Humans , Male , Aged , Bone Regeneration , /methods , Tooth Socket , Collagen , Esthetics, Dental , Mouth Rehabilitation/methodsABSTRACT
PURPOSE: This study was to evaluate the effects of bacterial cellulose (BC) membranes as a barrier membrane on guided bone regeneration (GBR) in comparison with those of the resorbable collagen membranes. MATERIALS AND METHODS: BC membranes were fabricated using biomimetic technology. Surface properties were analyzed, Mechanical properties were measured, in vitro cell proliferation test were performed with NIH3T3 cells and in vivo study were performed with rat calvarial defect and histomorphometric analysis was done. The Mann-Whitney U test and the Wilcoxon signed rank test was used (alpha<.05). RESULTS: BC membrane showed significantly higher mechanical properties such as wet tensile strength than collagen membrane and represented a three-dimensional multilayered structure cross-linked by nano-fibers with 60 % porosity. In vitro study, cell adhesion and proliferation were observed on BC membrane. However, morphology of the cells was found to be less differentiated, and the cell proliferation rate was lower than those of the cells on collagen membrane. In vivo study, the grafted BC membrane did not induce inflammatory response, and maintained adequate space for bone regeneration. An amount of new bone formation in defect region loaded with BC membrane was significantly similar to that of collagen membrane application. CONCLUSION: BC membrane has potential to be used as a barrier membrane, and efficacy of the membrane on GBR is comparable to that of collagen membrane.
Subject(s)
Animals , Rats , Biomimetics , Bone Regeneration , Cell Adhesion , Cell Proliferation , Cellulose , Collagen , Membranes , Osteogenesis , Porosity , Surface Properties , Tensile Strength , TransplantsABSTRACT
Os estudos abordando a regeneração dos tecidos dentários ganharam uma nova perspectiva com a utilização das células-tronco. E novas perspectivas têm surgido com a bioengenharia tecidual e as terapias periodontais e pulpares regeneradoras. O objetivo deste trabalho foi desenvolver o modelo experimental de autotransplante em ratos visando compará-lo à técnica de reimplante e estudar a capacidade terapêutica das células da medula óssea em diferentes biomateriais utilizados como matriz para a terapia de células-tronco no reparo dos tecidos dentais. Foram utilizados 23 ratos Wistar divididos em grupos de 1, 3, 15 e 60 dias para as técnicas de reimplante e autotransplante. Os grupos com injeção de células-tronco (CT) foram: (1) grupo de 3 dias, combinado à técnica de reimplante; (2) grupo de 15 dias com ambas as técnicas. Blocos contendo os três dentes molares superiores de cada lado dos ratos foram removidos, feitas radiografias periapicais e as peças foram processadas para inclusão em parafina. Foram avaliadas a espessura do ligamento periodontal (LPD) comparada entre os diferentes grupos e a morfologia celular e matriz extracelular relacionadas à superfície radicular, ao osso alveolar e à porção média do LPD, além das células da polpa dental de cada grupo. As células isoladas a partir da medula-óssea foram incubadas por 24h, 48h, e 72h em placas de cultura contendo membranas de colágeno bovino tipo I - CollaTape® (Integra LifeSciences Corporation, Plainsboro, NJ, USA), enxerto ósseo - Extra Graft XG-13® (Silvestre Labs Quimica e Farmaceutica LTDA, RJ, Brazil) ou um dente molar de rato. Os espécimes foram observados em um microscópio invertido para contagem de células e processadas para observação no microscópio eletrônico de varredura (MEV). Os grupos de 1 e 3 dias apresentaram medidas de LPD significativamente maiores para a técnica de autotransplante quando comparadas ao reimplante. O grupo de 3 dias com CT não apresentou alterações pulpares...
The studies on the regeneration of dental tissues have gained a new perspective with the use of stem cells. And new perspectives have appeared with bioengineering and pulp and periodontal regenerative therapies. The aim of this study was to develop an experimental model of autotransplantation in rats in order to compare it with tooth reimplant technique and to study the therapeutic potential of bone marrow cells in different biomaterials used as scaffolds for stem cell therapy to repair dental tissues. 23 rats divided in 1, 3, 15 and 60 days groups were used for the techniques of tooth reimplant and autotranplant. The groups with stem cell injection (CT) were: (1) 3 days, combined with tooth replant technique; (2) 15 days with both techniques. Blocks containing the three molar teeth from each side of the rats superior jaws were removed, periapical radiographs were taken and the specimens were processed and embedded in paraffin. LPD thickness among different groups and cell morphology and extracellular matrix related to the root surface, alveolar bone and the middle portion of the LPD, and dental pulp cells were evaluated and compared from each group. Cells isolated from bone marrow were incubated for 24h, 48h and 72h in culture plates containing membranes of bovine collagen type I - CollaTape ® (Integra LifeSciences Corporation, Plainsboro, NJ, USA), bone graft - Extra Graft XG-13 ® (Silvestre Labs Chemical and Pharmaceutical Ltda, RJ, Brazil) or a mouse molar tooth. The specimens were observed using an inverted microscope for cell count and processed for observation in a scanning electron microscope (SEM). Groups 1 and 3 days showed LPD thickness significantly higher for tooth autotransplant technique when compared to reimplant. The group of 3 days with stem cells showed no significant pulp changes different from control (without stem cells). Group of 15 days with stem cells showed the same histological characteristics of the group without injection...
Subject(s)
Animals , Rats , Bone Marrow Cells , Guided Tissue Regeneration, Periodontal , Stem Cells , Biocompatible Materials , Bone Transplantation , Periodontal Diseases , Periodontium , Rats, Wistar , Tooth Replantation , Transplantation, AutologousABSTRACT
Os estudos abordando a regeneração dos tecidos dentários ganharam uma nova perspectiva com a utilização das células-tronco. E novas perspectivas têm surgido com a bioengenharia tecidual e as terapias periodontais e pulpares regeneradoras. O objetivo deste trabalho foi desenvolver o modelo experimental de autotransplante em ratos visando compará-lo à técnica de reimplante e estudar a capacidade terapêutica das células da medula óssea em diferentes biomateriais utilizados como matriz para a terapia de células-tronco no reparo dos tecidos dentais. Foram utilizados 23 ratos Wistar divididos em grupos de 1, 3, 15 e 60 dias para as técnicas de reimplante e autotransplante. Os grupos com injeção de células-tronco (CT) foram: (1) grupo de 3 dias, combinado à técnica de reimplante; (2) grupo de 15 dias com ambas as técnicas. Blocos contendo os três dentes molares superiores de cada lado dos ratos foram removidos, feitas radiografias periapicais e as peças foram processadas para inclusão em parafina. Foram avaliadas a espessura do ligamento periodontal (LPD) comparada entre os diferentes grupos e a morfologia celular e matriz extracelular relacionadas à superfície radicular, ao osso alveolar e à porção média do LPD, além das células da polpa dental de cada grupo. As células isoladas a partir da medula-óssea foram incubadas por 24h, 48h, e 72h em placas de cultura contendo membranas de colágeno bovino tipo I - CollaTape® (Integra LifeSciences Corporation, Plainsboro, NJ, USA), enxerto ósseo - Extra Graft XG-13® (Silvestre Labs Quimica e Farmaceutica LTDA, RJ, Brazil) ou um dente molar de rato. Os espécimes foram observados em um microscópio invertido para contagem de células e processadas para observação no microscópio eletrônico de varredura (MEV). Os grupos de 1 e 3 dias apresentaram medidas de LPD significativamente maiores para a técnica de autotransplante quando comparadas ao reimplante. O grupo de 3 dias com CT não apresentou alterações pulpares ...
The studies on the regeneration of dental tissues have gained a new perspective with the use of stem cells. And new perspectives have appeared with bioengineering and pulp and periodontal regenerative therapies. The aim of this study was to develop an experimental model of autotransplantation in rats in order to compare it with tooth reimplant technique and to study the therapeutic potential of bone marrow cells in different biomaterials used as scaffolds for stem cell therapy to repair dental tissues. 23 rats divided in 1, 3, 15 and 60 days groups were used for the techniques of tooth reimplant and autotranplant. The groups with stem cell injection (CT) were: (1) 3 days, combined with tooth replant technique; (2) 15 days with both techniques. Blocks containing the three molar teeth from each side of the rats superior jaws were removed, periapical radiographs were taken and the specimens were processed and embedded in paraffin. LPD thickness among different groups and cell morphology and extracellular matrix related to the root surface, alveolar bone and the middle portion of the LPD, and dental pulp cells were evaluated and compared from each group. Cells isolated from bone marrow were incubated for 24h, 48h and 72h in culture plates containing membranes of bovine collagen type I - CollaTape ® (Integra LifeSciences Corporation, Plainsboro, NJ, USA), bone graft - Extra Graft XG-13 ® (Silvestre Labs Chemical and Pharmaceutical Ltda, RJ, Brazil) or a mouse molar tooth. The specimens were observed using an inverted microscope for cell count and processed for observation in a scanning electron microscope (SEM). Groups 1 and 3 days showed LPD thickness significantly higher for tooth autotransplant technique when compared to reimplant. The group of 3 days with stem cells showed no significant pulp changes different from control (without stem cells). Group of 15 days with stem cells showed the same histological characteristics of the group without injection ...
Subject(s)
Animals , Rats , Bone Marrow Cells , Guided Tissue Regeneration, Periodontal , Stem Cells , Biocompatible Materials , Bone Transplantation , Periodontal Diseases , Periodontium , Rats, Wistar , Tooth Replantation , Transplantation, AutologousABSTRACT
The ultimate goal of periodontal therapy is to repair the damaged periodontal supporting tissues, permitting regeneration of the periodontal ligament. However, the cell response, the supportive matrix and the bioactive molecules use have not yet been well established. Bone marrow mononuclear cells were extracted from rat femurs and tibiae and cultured on a cross-linked collagen membrane, bone graft, or molar tooth to compare cell attachment and early proliferation on these materials. Cell attachment was quantified by light microscopy at 24, 48 and 72h, and cell proliferation was observed under a SEM after 72h. After 24h, the number of cells on bone graft was similar to that of the control and more than twice compared to collagen membrane (q=7.473 p<0.001) and 1.75 times greater than with tooth cementum (q=5.613 p<0.01). However, the number of cells close to bone graft decreased in the second day compared to the control. SEM examination revealed a significant decrease in the number of cells that attached and proliferated on tooth and bone graft when compared with membrane. The results showed that bone marrow mesenchymal cells offer great potential for colonize a collagen membrane.
El objetivo último de la terapia periodontal es reparar el daño tejidos periodontales de soporte, permitiendo la regeneración del ligamento periodontal. Sin embargo, la respuesta de la célula, la matriz de apoyo y las moléculas bioactivas aún no han sido bien establecidas. Células mononucleares de la médula ósea se extrajeron del fémur y fibula de rata, y fueron cultivadas sobre un reticulado de membrana de colágeno, de injerto de hueso o de un diente molar para comparar la adhesión celular y la proliferación temprana sobre estos materiales. La adhesión celular fue cuantificada por microscopía de luz a las 24, 48 y 72h, y la proliferación celular fue observada bajo MEB después de 72h. Después de 24 horas, el número de células sobre el injerto de hueso fue similar a la del control y más del doble en comparación con la membrana de colágeno (q=7,473 p<0,001) y 1,75 veces mayor que con el cemento dental (q=5,613 p<0,01). Sin embargo, el número de células cerca del injerto óseo disminuyó el segundo día en comparación con el control. El examen al MEB reveló una disminución significativa en el número de células que se unen y proliferan sobre los dientes y el injerto óseo en comparación con la membrana. Los resultados mostraron que las células mesenquimales de la médula ósea tienen un gran potencial para colonizar la membrana de colágeno.